TABLE 2.
Cell dry weights and CGP contents determined from cultivations under different conditionsa
| Strain(plasmid) | Growth condition variable | CDM (mg) | % CGP content (mean ± SD)
|
|
|---|---|---|---|---|
| Insoluble | Soluble | |||
| G175(pESC-URA) | 85 | - | - | |
| G175(pYEX-BX) | Plus 0.1 mM CuSO4 | 106 | - | - |
| BY4741(pYEX-BX) | Plus 0.1 mM CuSO4 | 81 | - | - |
| G175(pESC-URA::cphA6308) | 81 | 0.1 ± 0.1 | 1 ± 1.0 | |
| Plus glycerol 3% (wt/wt) | 82 | 0.1 ± 0.1 | 1 ± 1.0 | |
| YPD medium | 249 | 0.1 ± 0.1 | - | |
| Plus 10 mM Asp, 15 mM Arg | 99 | 0.3 ± 0.1 | - | |
| Plus 15 mM Arg | 98 | 1.2 ± 0.2 | - | |
| Plus 15 mM Lys | 97 | 1.4 ± 0.2 | - | |
| G175(pYEX-BX::cphA6308) | Without CuSO4 | 88 | 1.3 | 3.1 |
| Plus 0.1 mM CuSO4 | 85 | 2.5 ± 1.0 | 3.7 ± 0.2 | |
| Plus 0.2 mM CuSO4 | 85 | 2.2 ± 0.2 | 4.7 ± 0.2 | |
| Plus 0.4 mM CuSO4 | 83 | 1.5 ± 0.2 | 4.2 ± 0.3 | |
| Plus 0.1 mM CuSO4; YPD medium | 288 | 0.1 ± 0.1 | - | |
| Plus 0.1 mM CuSO4, 10 mM Asp, and 15 mM Arg | 129 | 2.7 ± 0.2 | - | |
| Plus 0.1 mM CuSO4 and 15 mM Arg | 121 | 3.1 ± 0.2 | - | |
| Plus 0.1 mM CuSO4 and 15 mM Lys | 83 | 2.2 ± 0.2 | - | |
| BY4741(pYEX-BX::cphA6308) | Without CuSO4 | 61 | 0.7 | 3.0 |
| Plus 0.1 mM CuSO4 | 46 | 2.0 ± 1.0 | 3.9 ± 0.2 | |
| Plus 0.2 mM CuSO4 | 39 | 0.6 ± 0.2 | 3.9 ± 0.3 | |
| Plus 0.4 mM CuSO4 | 37 | 0.6 ± 0.1 | 4.2 ± 0.1 | |
| Plus 0.1 mM CuSO4; YPD medium | 227 | 0.3 ± 0.1 | - | |
| Plus 0.1 mM CuSO4, 10 mM Asp, and 15 mM Arg | 78 | 3.9 ± 0.4 | - | |
| Plus 0.1 mM CuSO4 and 15 mM Arg | 75 | 3.3 ± 0.4 | - | |
| Plus 0.1 mM CuSO4 and 15 mM Lys | 71 | 3.2 ± 0.4 | - | |
Cell dry weights and CGP contents are the results of cultivating S. cerevisiae strains BY4741 and G175 harboring vector pESC-URA or pYEX-BX or pESC-URA::cphA6308 and pYEX-BX::cphA6308, respectively, under different conditions. All strains were grown at 30°C for 48 h. Strains harboring pESC-URA and pESC-URA::cphA6308 were grown in minimal medium containing 2% (wt/vol) galactose except where YPD medium is listed. Strains harboring pYEX-BX::cphA6308 were grown in minimal medium containing 2% (wt/vol) glucose except where YPD medium is listed. Cell dry matter (CDM) amounts were determined as described in Materials and Methods. Insoluble and soluble CGP samples were isolated as described in Materials and Methods. Most cultivations were performed at least three times. Values given are means ± standard deviations (SD). -, CGP could not be isolated.