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Levels of substratum coverage by E. coli SAR19 biofilms formed on glass (open symbols) or vinyl plastic (filled symbols) substrata. The surfaces were either preconditioned with the total extract (triangles) or the isoelectric fraction (squares) or left uncoated (circles). The biofilms were grown in minimal medium in flow cells at 30°C. Standard deviations for four distinct channels are indicated.
