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. 2008 Mar 31;74(11):3323–3327. doi: 10.1128/AEM.00060-08

TABLE 1.

Biochemical profiles and sequence identification of nonfermentative bacterial isolates from oysters and seawater from the Delaware Bay

Stock or source API codea Sequence identificationb Growth on 6% NaClc H2S on TSId Hemolysise Growth atf:
25°C 37°C 42°C
Stock cultures
    Shewanella algae 0402004 Shewanella algae + + α + + +
    Shewanella algidipiscicola 0400004 Shewanella algidipiscicola − (25°C) + − (25°C) + - -
    Shewanella baltica 0502004 Shewanella baltica − (25°C) + − (25°C) + - -
    Shewanella frigidamarina 0400004 Shewanella frigidimarina − (25°C) - − (25°C) + - -
    Shewanella morhuae 0402004 Shewanella morhuae − (25°C) - − (25°C) + - -
    Shewanella putrefaciens 0000004 Shewanella putrefaciens - - α + + -
Environmental samples
    Oysters 1402004 Shewanella abalonesis (99%) + + α + + -
    Seawater 0502004 Shewanella abalonesis (>98%) + + α + + +
    Oysters 0402004 Shewanella algae - + β + + +
    Oysters 0402004 Shewanella baltica (>99%) - + α + + -
    Seawater 0002004 Shewanella baltica - - β + + -
    Oysters 0002004 Shewanella baltica - + α + + -
    Oysters 0000004 Shewanella hafniensis (99%) - - - + + -
    Oysters 0400004 Shewanella marisflavi (>99%) - + α + + -
    Oysters 0400004 Shewanella marisflavi (>99%) - + α + + -
    Oysters 0002004 Shewanella putrefaciens (>99%) - + α + + -
    Oysters 0400004 Photobacterium damselae subsp. damselae - + α + + -
    Oysters 0402004 Photobacterium damselae subsp. damselae - + β + + +
    Oysters 1040004 Photobacterium damselae subsp. damselae + - α + + -
    Oysters 0000004 Listonella anguillarum - + α + + -
    Oysters 0402004 Listonella anguillarum - + α + + -
    Oysters 0000004 Listonella anguillarum - + α + + -
    Oysters 0002004 Listonella anguillarum - + α + + -
    Oysters 0000004 Listonella anguillarum - - α + + -
    Seawater 0002004 Listonella anguillarum - - β + + -
    Seawater 0402004 Listonella anguillarum - + α + + -
    Oysters 1002004 Listonella anguillarum + - - + + -
    Seawater 1000004 Listonella anguillarum + - α + + -
    Oysters 1040004 ND + - α + + -
    Oysters 0402004 ND - + α + + -
    Oysters 0002004 ND - - α + + -
a

Bold type signifies API 20E identification of Shewanella putrefaciens (often in error), with API codes of 0502004 indicating an excellent ID, 0402004 indicating a very good ID, and 0400004 indicating an acceptable ID, according to the API database. Specific biochemical reactions for these codes are shown in Table 2.

b

Identification based on 16S rRNA gene at 100% sequence identity (or other percentage indicated in parentheses). ND, sequence not determined.

c

Growth on tryptic soy agar containing 6% total NaCl in 24 h at 37°C or, if noted in parentheses, at 25°C.

d

H2S produced from TSI in 24 h at 25°C.

e

Alpha or beta hemolysis on blood agar plates in 24 h at 37°C or, if noted in parentheses, at 25°C. A minus (−) indicates no hemolysis.

f

Growth within 24 h on tryptic soy agar containing 1% total NaCl at various incubation temperatures.