FIG. 10.

Indirect immunofluorescence assay. Thin smears of washed P. vivax-infected erythrocytes were prepared and incubated with preimmune rabbit serum as a negative control on the early schizont stage of the parasite (a) and with polyclonal antibody raised against PvTRAg40 (b, c, and d [ring, trophozoite, and schizont stages of the parasite, respectively]), followed by a fluorescein isothiocyanate-conjugated secondary antibody. Parasitized red blood cells are indicated by arrows. The same fields of cells were examined by fluorescence (left panels) and phase-contrast (right panels) microscopy, at a magnification of ×100.