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Levels of IgG1 and IgG4 antibodies against PvTRAg40 in a group of recovered patients. ELISA was performed on serum samples (n = 24) from individuals who had been exposed to P. vivax infection 8 to 14 weeks prior to sample collection, using HPLC-purified PvTRAg40 and biotin-labeled monoclonal anti-human IgG1 or IgG4. The levels of IgG4 antibodies were significantly higher than those of IgG1 (P < 0.05).
