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. 2008 Mar 17;76(6):2541–2550. doi: 10.1128/IAI.01310-07

FIG. 2.

FIG. 2.

IL-2 secretion by Jurkat cells infected with wild-type B. pseudomallei, bsaQ and fliC B. pseudomallei mutants, E. coli DH5α, or E. coli ΔfliC mutant or incubated with bacterial culture supernatant. A total of 2 × 106 Jurkat cells were uninfected or infected with KHW or KHWbsaQ mutant at MOIs of 1, 10, and 30 (A) or with KHW, KHWfliCKm, or KHWfliCpro at MOIs of 5, 30, and 100 (C) or with DH5α or ΔfliC mutant (E) for 2 h, followed by 22 h of exposure to 2.5 μg of plate-bound anti-CD3 antibody/ml. In some experiments, 0.2 × 106 Jurkat cells were incubated in anti-CD3 coated plate for 22 h, together with LB medium or log-phase bacterial culture supernatants of KHW, KHWbsaQ (B), KHWfliCKm and KHWfliCpro (D), or E. coli DH5α or ΔfliC mutant (F), which were filter sterilized. The results from one representative experiment out of three are shown, and error bars represent the standard deviation of three values.