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Detection of flagellin in KHW cell lysate and supernatant but not in fliC mutants. (A) Western blot analysis of flagellin protein in protein precipitates prepared from filter-sterilized bacterial culture supernatant of KHW (lane 1) and KHWfliCKm (lane 2). Lane 3 contained 1 μg of recombinant B. pseudomallei flagellin protein. (B) Western blot analysis of flagellin protein in KHW (lane 1) and KHWfliCpro (lane 2) lysate.
