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. 2008 Apr 14;76(6):2736–2745. doi: 10.1128/IAI.01085-07

FIG. 9.

FIG. 9.

Identification of proteins and carbohydrates present in A. suum extract. Silver-stained 8 to 25% polyacrylamide gradient electrophoresis gel of A. suum extract (A) and a toluidine blue-stained 0.6% agarose gel for carbohydrate staining (B). Lane 1, low-molecular-mass markers (97-kDa phosphorylase b, 66-kDa albumin, 45-kDa ovalbumin, 30-kDa carbonic anhydrase, 20.1-kDa trypsin inhibitor; 14.4-kDa α-lactoalbumin); lanes 2, 3, and 4, 0.5, 1, and 2 μg of the A. suum extract, respectively; lane 5, high-molecular-mass markers (220-kDa thyroglobulin, 170-kDa ferritin, 116-kDa catalase, 76-kDa lactase dehydrogenase, and 53-kDa albumin). (B) Lane 1, chondroitin-4-sulfate; lane 2, chondroitin-6-sulfate; lane 3, 50 μg A. suum. Experiments were done three times.