FIG. 6.

Increased expression of Ehformin-1 and -2 delays cell division. (A) Forty-eight-hour-grown cells of Ehformin-1, Ehformin-2, and control transformants maintained at 40 μg/ml G418 were ethanol fixed, DAPI stained, and counted for the number of nuclei in each cell. Percentages of uninucleated, binucleated, and multinucleated (more than 2 nuclei per cell) cells were plotted on a bar graph. Error bars, standard deviations (n = 3). (B) Representative images of DAPI-stained cells from control and Ehformin-1 transformants maintained at 40 μg/ml G418. In addition to uninucleated and binucleated cells, cells containing multiple (3 to 8) nuclei are often seen in both Ehformin-1 and Ehformin-2 transformants, while multinucleated cells in control transformants contain only 2 to 3 nuclei. Arrows indicate DAPI-stained nuclei that are out of focus in the Z-section shown here. Bar, 20 μm.