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. 2008 Mar 17;76(6):2368–2378. doi: 10.1128/IAI.01449-07

FIG. 7.

FIG. 7.

Increased expression of Ehformin-1 and -2 leads to increases in the DNA contents of E. histolytica nuclei. Forty-eight-hour-grown cells of Ehformin-1, Ehformin-2, and control transformants maintained at 10, 20, and 40 μg of G418/ml were stained with DAPI to analyze the DNA contents of individual nuclei in a Metacyte scanning cytometer using Metafer4. (a to c) Normalized DAPI fluorescence values for nuclei from cells of control (a), Ehformin-1 (b), and Ehformin-2 (c) transformants maintained at different G418 concentrations (10, 20, and 40 μg/ml) were plotted as individual histograms. (d) Histograms corresponding to the set of nuclei from control, Ehformin-1, and Ehformin-2 transformants maintained at 40 μg of G418/ml.