TABLE 1.

Summary of anti-fusion protein antibody reactivity

Antibodya	Reactivity with tagged fusion protein in indicated assayb
	RFP		GST (WB)
	IFA	WB
Anti-CT005	+	ND	+
Anti-CT006	−	+	+
Anti-CT036	+	ND	+
Anti-CT058	+	ND	+
Anti-CT089	+	ND	+
Anti-CT101	+	ND	+
Anti-CT115	+	ND	+
Anti-CT116	+	ND	+
Anti-CT117	+	ND	+
Anti-CT118	+	ND	+
Anti-CT119	+	ND	+
Anti-CT134	−	−	+c
Anti-CT135	+	ND	+
Anti-CT147	+	ND	+
Anti-CT164	−	+	+
Anti-CT179	+	ND	+
Anti-CT192	+	ND	+
Anti-CT195	+	ND	+
Anti-CT196	−	+	+
Anti-CT214	+	ND	+
Anti-CT222	+	ND	+
Anti-CT223	+	ND	+
Anti-CT224	+	ND	+
Anti-CT225	+	ND	+
Anti-CT226	+	ND	+
Anti-CT227	−	−	+c
Anti-CT228	+	ND	+
Anti-CT229	+	ND	+
Anti-CT232	+	ND	+
Anti-CT233	+	ND	+
Anti-CT249	+	ND	+
Anti-CT288	+	ND	+
Anti-CT300	−	+	+
Anti-CT345	+	ND	+
Anti-CT357	+	ND	+
Anti-CT358	+	ND	+
Anti-CT365	−	−	+c
Anti-CT383	+	ND	+
Anti-CT440	+	ND	+
Anti-CT442	+	ND	+
Anti-CT449	+	ND	+
Anti-CT483	−	−	+c
Anti-CT484	+	ND	+
Anti-CT529	+	ND	+
Anti-CT565	+	ND	+
Anti-CT618	+	ND	+
Anti-CT728	+	ND	+
Anti-CT789	−	−	+c
Anti-CT813	+	ND	+
Anti-CT850	+	ND	+

^aMouse antibodies raised with GST fusion proteins.

^bRFP and GST were tagged to the N termini of chlamydial proteins. IFA, immunofluorescence assay; WB, Western blot assay; +, positive reaction; −, negative reaction; ND, not detected.

^cThese five antibodies only reacted with the corresponding GST, but not RFP, fusion proteins, suggesting that these antibodies cannot recognize C. trachomatis-specific proteins.