TABLE 2.
Summary of immunofluorescence detection of 50 C. trachomatis proteins in infected cells using mouse anti-fusion protein antibodies in comparison with previously known information
| Location of protein and ORF | Previous evidencea | Computer predictionb
|
|
|---|---|---|---|
| Ban | Toh | ||
| Inclusion membrane | |||
| CT089 | IM+ | − | − |
| CT115 | IM+ | + | + |
| CT116 | IM+ | + | + |
| CT117 | IM+ | + | + |
| CT118 | IM+ | + | + |
| CT119 | IM+ | + | + |
| CT147 | IM+ | − | + |
| CT223 | IM+ | + | + |
| CT225 | − | + | + |
| CT226 | IM+ | + | + |
| CT228 | − | + | + |
| CT229 | IM+ | + | + |
| CT232 | IM+ | + | + |
| CT233 | IM+ | + | + |
| CT249 | IM+ | − | + |
| CT288 | IM+ | + | + |
| CT358 | − | + | + |
| CT440 | − | + | + |
| CT442 | IM+ | + | − |
| CT529 | IM+ | − | − |
| CT618 | IM+ | + | − |
| CT813 | IM+ | + | + |
| Intrainclusion | |||
| CT058 | − | + | + |
| CT192 | − | + | + |
| CT195 | − | + | + |
| CT383 | − | + | + |
| CT484 | IB+ | + | + |
| CT565 | − | + | − |
| CT850 | − | + | − |
| Undefinedc | |||
| CT005 | − | + | − |
| CT006 | − | + | − |
| CT036 | − | + | + |
| CT101 | − | + | − |
| CT134 | − | + | − |
| CT135 | − | + | − |
| CT164 | − | + | − |
| CT179 | − | − | + |
| CT196 | − | + | + |
| CT214 | − | + | + |
| CT222 | − | − | + |
| CT224 | − | + | + |
| CT227 | − | + | + |
| CT300 | − | + | + |
| CT345 | − | + | + |
| CT357 | − | + | + |
| CT365 | − | − | + |
| CT449 | − | + | + |
| CT483 | − | + | + |
| CT728 | − | + | − |
| CT789 | − | + | − |
Previous experimental evidence for intracellular location of the 50 proteins is listed in this column. Previously described proteins and the references in which their localizations are described are CT089 (14); CT115 to -119 (35); CT147 (6); CT223, -229, -233, -288, -442, and -484 (2); CT226 (38); CT229 (30); CT232 (IncB) and CT233 (IncC) (3); CT249 (22); CT442 (44); CT529 (15); CT618 (42); and CT813 (8). IM+, positive localization in the inclusion membrane; IB+, positive localization inside the inclusions; −, published experimental evidence for the intracellular location of the corresponding proteins is lacking.
Ban and Toh stand for references 2, Bannantine et al., and 49, Toh et al., respectively. +, positive prediction for inclusion membrane localization; −, negative prediction for inclusion membrane localization.
No specific signals were detected using the corresponding antibodies under the conditions in which the experiments were carried out.