TABLE 1.
Promoter and regiona | % Activityb
|
|||
---|---|---|---|---|
Hi.Shk | Hi.Std | Lo.Shk | Lo.Std | |
WT acr | 0.06 | 91.60 | 19.67 | 81.53 |
SR1 | Bg | 72 | 214 | 63 |
SR2 | Bg | 77 | 138 | 35 |
M1 | Bg | 23 | 25 | 8 |
M0 | Bg | 28 | 17 | 5 |
M2 | Bg | 6 | 2 | 1 |
M3 | Bg | 1 | 2 | 1 |
SR3 | Bg | 61 | 83 | 58 |
SR4 | Bg | 7 | 9 | 1 |
SR5 | Bg | 977 | 2,484 | 1,013 |
WT acg | 0.17 | 146.88 | 25.46 | 62.97 |
SR5 | Bg | 623 | 250 | 411 |
SR4 | Bg | 170 | 182 | 95 |
SR3 | Bg | 80 | 61 | 64 |
M3 | Bg | 1 | 2 | 0 |
M2 | Bg | 4 | 3 | 5 |
M0 | Bg | 0 | 5 | 1 |
M1 | Bg | 0 | 1 | 1 |
SR2 | Bg | 34 | 36 | 24 |
SR1 | Bg | 66 | 261 | 88 |
All values are presented relative to the WT M. tuberculosis acr or acg promoter sequence. SRs are as defined in the text. Regions in boldface correspond to specific ACG motif copy replacements.
Conditions were as described in the text. Only ACG-inducing conditions are shown, as all promoter levels were at background (Bg) under the noninducing, shaking, high-oxygen conditions.