FIG. 2.

Western immunoblot analysis of P1ΔP reconstituted with P region repeats. Recombinant P1 polypeptides were separated on replicate 10% SDS-polyacrylamide gels, and the corresponding nitrocellulose membranes were reacted with the indicated anti-P1 polyclonal and monoclonal reagents. Lanes a through f contain samples derived from E. coli harboring the pUC18 vector-only control and plasmids pDC9 (P1ΔP), pDC9-7 (one repeat), pDC9-15 (two repeats), pDC9-22 (three repeats), and pDC20 (containing unaltered spaP), respectively. The migration of molecular mass standards is indicated on the left of the upper panels, and the apparent molecular masses of the full-length P1 and P1ΔP polypeptides are indicated on the right.