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. 2008 Mar 24;76(6):2520–2530. doi: 10.1128/IAI.01652-07

FIG. 2.

FIG. 2.

Genetic organization of the M. catarrhalis hfq chromosomal locus. (A) Schematic representation of the M. catarrhalis hfq chromosomal locus in M. catarrhalis ATCC 43617, including the two genes flanking the hfq ORF. The relative positions of the different oligonucleotide primers used for PCR and RT-PCR are indicated by the arrows. (B) Photograph of an agarose gel showing the results of a RT-PCR experiment involving the hfq ORF and the two flanking genes. The primer pair used in each experiment is indicated on the top of the gel. Lane 1 contains DNA size markers. Lanes 2 and 5 contain samples from RT-PCRs where DNA was used as the template. Lanes 3 and 6 contain samples from RT-PCRs where RNA was used as the template but no reverse transcriptase was added [(−)RT]. Lanes 4 and 7 contain samples from RT-PCRs where RNA was used as the template and reverse transcriptase was added [(+)RT].