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. 2008 Mar 24;76(6):2520–2530. doi: 10.1128/IAI.01652-07

FIG. 3.

FIG. 3.

Construction of M. catarrhalis hfq mutants. (A and B) Schematic representation of the M. catarrhalis chromosomal locus containing the hfq gene and flanking regions in the hfq mutant O35E.hfq::kan (A) and the hfq deletion mutant O35EΔhfq (B). The relative positions of the different primers used for PCR are indicated by the arrows. (C) Western blot analysis using mouse polyclonal antibody raised against the recombinant M. catarrhalis O35E Hfq protein to probe WCL derived from the wild-type O35E strain (WT, lane 1), the O35EΔhfq mutant (Δhfq, lane 2), the O35EΔhfq mutant containing the pAA200 plasmid with the wild-type O35E hfq gene [Δhfq(pAA200), lane 3], and this same mutant containing only the plasmid vector [Δhfq(pWW115), lane 4].