FIG. 8.
The M. catarrhalis hfq mutant exhibits a growth advantage in a biofilm system. Cells of M. catarrhalis O35E-Smr (▪) and O35E.hfq::kan (□) were mixed together (input) and used to inoculate MH broth in a flask (A) and a Sorbarod filter in silicone tubing that was supplied with a continuous flow of MH broth (B). The cultures were allowed to grow overnight at 37°C and then harvested (output), serially diluted, and plated onto agar media containing the appropriate antibiotics to determine the relative percentages of each strain in the mixture. The data presented are the means of three independent experiments.