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. 2008 Mar 31;76(6):2341–2351. doi: 10.1128/IAI.01600-07

FIG. 3.

FIG. 3.

TLR2, TLR5, and TLR9 do not participate in the phagocytosis of B. burgdorferi. (A) Wild-type (WT) control or TLR2−/− BMDM were plated in 24-well plates and infected with B. burgdorferi spirochetes (Bb) for 5 min, 20 min, and 60 min. (B) Raw 264.7 cells were transfected with either control or TLR5 siRNAs, and after 24 h, the cells were infected with B. burgdorferi for 5 min, 20 min, and 60 min. (C) Wild-type control or TLR9−/− BMDM were plated in 24-well plates and infected with B. burgdorferi for 5 min, 20 min, and 60 min. The phagocytosis assay and staining were performed as described in the legend to Fig. 1. Cells containing internalized B. burgdorferi particles were counted and expressed as a percentage of the total number of cells examined. The data are representative of three or four independent experiments. The error bars represent standard deviations.