FIG. 4.

Extracellular SerB regulates F actin in gingival epithelial cells. HIGK cells were reacted with rSerB or rPG1170. The control (Ctrl) was prepared in the absence of phosphatase. (A) Actin was visualized with rhodamine-phalloidin (red), and bacterial phosphatases were visualized with SerB antibody and fluorescein-labeled secondary antibody (green). Confocal x-y projections of a z-stack (×40) are shown, along with x-z and y-z projections for the merged images (×60) with enzyme-reacted cells. (B) F/G actin ratio in HIGKs reacted with SerB. Error bars represent standard deviations. Values are statistically different at P < 0.01 (t test).