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Gating scheme for cell identification and intracellular cytokine staining. (A) Gating scheme used for identification of T cells and characterization of intracellular cytokine expression. Staining of spleen cells from mice 7 days after intradermal inoculation is shown. (B) Cellular characterization of neutrophils, alveolar macrophages, dendritic cells, and macrophages in the bronchoalveolar spaces. Staining of BAL cells from mice 10 days post-intranasal inoculation is shown.
