TABLE 1.

Cellular infiltration into the bronchoalveolar space after intradermal or intranasal inoculation

Daye	Groupc	Cell countab
		CD19+	CD11c+	CD11c+ CD11blow GR1mid	CD11b+	CD11bhi GR1hi	NK1.1+
0	Control	18 ± 5	764 ± 213	11,000 ± 1,800	13 ± 3	152 ± 35	340 ± 262
7	ID	7 ± 7	875 ± 55	13,000 ± 2,300	19 ± 7	111 ± 26	1725 ± 620d
	IN	40 ± 10	2,291 ± 270d	7,600 ± 1,200	1,083 ± 147d	5,772 ± 808d	698 ± 69
10	ID	13 ± 1	996 ± 211	4,600 ± 770	16 ± 4	193 ± 27	376 ± 126
	IN	316 ± 107d	5,593 ± 665d	7,300 ± 130	1,870 ± 361d	8,937 ± 1375d	2,647 ± 253d
14	ID	17 ± 4	836 ± 103	4,300 ± 660	17 ± 2	240 ± 36	84 ± 18
	IN	9,644 ± 4,677d	3,334 ± 1,670	10,000 ± 4,400	2,351 ± 1,362d	3,452 ± 1,362d	950 ± 586

^aValues are means ± standard errors (eight samples per group).

^bCells were defined as B cells (CD19⁺), DCs (CD11c⁺), alveolar macrophages (CD11c⁺ CD11b^low GR1^mid), macrophages (CD11b⁺ CD11c⁻), neutrophils (CD11b^hi GR1^hi), or NK cells (NK1.1⁺).

^cID, intradermally inoculated mice; IN, intranasally inoculated mice.

^dSignificant difference (P ≤ 0.05) between results for routes of inoculation at each time point as calculated by Student's t test. Data represented are combined from two experiments with four mice per group for a total of eight mice per group per time point.

^eDay postinoculation. “0” indicates mock inoculation.