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. 1997 Nov 25;94(24):12875–12879. doi: 10.1073/pnas.94.24.12875

Figure 1.

Figure 1

Z-DNA cleavage by Zα nuclease. (A) FokI has a modular structure with two separable domains, the DNA recognition domain and the nuclease cleavage domain. The Zα domain of hADAR1 replaces the DNA recognition domain of FokI and is linked to the cleavage domain through a (Gly4Ser)3 linker (curved line). (B) Zα nuclease cleavage of the parental plasmid, pDPL6, and pDHg16 containing a (dC-dG)13 insert in relaxed or negatively supercoiled form. This reaction is followed by PstI digestion. The reactions were electrophoresed in a 1% agarose gel. Linear DNA (2.2 kb) and the Zα nuclease cleaved DNA products (1.4 and 0.8 kb) are indicated by arrows. The BssHII restriction enzyme digestion of pDHG16 plasmid linearized with PstI shows the boundaries of the (dC-dG)13. This reaction yields similar-sized fragments to that of Zα nuclease digestion.