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. 1997 Nov 25;94(24):12875–12879. doi: 10.1073/pnas.94.24.12875

Figure 2.

Figure 2

Primer extension of the two cleavage product pDHg16 with Thermo Sequenase with either an EcoRI primer (A) or a SalI primer (B). The reactions were electrophoresed in a 7% denaturing polyacrylamide gel. Sequencing reactions (G A T C) of pDHg16 with each primer are shown beside the corresponding primer extension reactions (cut). Arrows indicate the cleavage sites and intensities. The thick vertical line indicates the location of (dC-dG)13 insert. (C) The position of the cleavage sites are mapped by vertical arrows.