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. 2008 Mar 31;76(6):2620–2632. doi: 10.1128/IAI.01308-07

FIG. 5.

FIG. 5.

The cytotoxicity of V. anguillarum M93Sm and hemolysin mutant strains against ASK cells. (A) ASK cells were treated with M93Sm and its derivative hemolysin mutants at an MOI of 500 for 4 h. Determination of the ASK cell density and viability is described in Materials and Methods and was carried out using a trypan blue dye exclusion assay. Bars represent the standard deviations of three independent measurements. P values above each bar of assays were calculated by t test analysis. (B) The morphological changes of treated ASK cells were observed by inverted microscopy at a magnification of ×100. ASK cells were incubated for 1 h with NSS-washed V. anguillarum cells at an MOI of 100. The V. anguillarum strains added were M93Sm (B-1), S171 (vah1 mutant) (B-2), S123 (rtxA mutant) (B-3), S183 (vah1 rtxA double mutant) (B-4), and mock (NSS).