FIG. 1.

Schematic model of fHbp chimera I. The A domain (not shown) and N-terminal portion of the B domain (right, dark gray) are encoded by the fHbp gene from strain MC58 and express the v.1 epitopes recognized by anti-fHbp MAbs, JAR 3 and JAR 5. The carboxy-terminal portion of the B domain and the C domain (left, light gray) are encoded by the gene from strain 8047 and contain epitopes recognized by JAR 10, 11, 13, and 36 (v.2 and v.3). The junction point between the two portions of the chimera is residue G136, which immediately precedes the alpha-helix. Chimera II differs from chimera I in having the A174K substitution, which eliminated the JAR 11 epitope and introduced the JAR 32 and JAR 35 epitopes. The coordinates were from the nuclear magnetic resonance solution structure of the combined B and C domains of fHbp v.1 (7). The amino terminus (residue 109) and carboxyl terminus (residue 255) are labeled N and C, respectively.