TABLE 1.

Strains, plasmids, and primers

Strain, plasmid, or primer	Genotype, description, or sequence	Reference or source
Strains
RN6390	WT laboratory strain	35
ALC42	DB clinical isolate	12
ALC32	DB sarA::Tn917	12
SMC1062	MZ100 laboratory strain isogenic to RN6390	61
SMC2715	MZ100 sarA::aphA-3	61
ALC136	RN6390 sarA::Tn917	13
ALC600	RN6390 sarA::Tn917 pCL84-sarA	11
SMC2713	MZ100 ica::tetK	61
ALC2629	RN6390 fnbA::tet	This study
ALC2634	RN6390 fnbB::erm	This study
CMS392	RN6390 ΔgraR	51
CMS399	RN6390 ΔgraS	51
CMS400	RN6390 ΔvraG	51
ALC4301	Mu50 (VISA)	39
ALC5908	Mu50 ΔgraR	51
ALC960	Col (methicillin-resistant S. aureus)	26
ALC5542	Col ΔgraR	51
SH1000	rsbU+	33
SH1000 acn	acn	Simon Foster
ALC1745	RN6390 pALC1484	47
ALC1747	RN6390 pALC1747	73
Plasmids
pEPSa5	pC194-based expression vector	22
pEPSAgraR	pEPSA5 with graR gene	51
pEPSAgraRS	pEPSA5 with graRS genes	51
pALC1484	pSK236 plasmid with promoterless gfp	47
pALC1747	fnbA-gfp transcription fusion derived from pALC1484	73
Primersa
gyrBsybrF	GGTGCTGGGCAAATACAAGT
gyrBsybrR	TGGGATACCACGTCCGTTAT
F-fnbA-rt	ACAAGTTGAAGTGGCACAGCC
R-fnbA-rt	CCGCTACATCTGCTGATCTTGTC
F-fnbB-rt	CACCGAAAACTGTGCAAGCA
R-fnbB-rt	TTCCTGTAGTTTCCTTATCAGCAACTT

^aPrimers were used for qRT-PCR.