TABLE 2.
Oxidative damage in tissues as measured by 8-isoprostane levelsa
| Tissue type | Mean 8-isoprostane level (pg/ml) ± SEM for indicated age and genotype
|
||||
|---|---|---|---|---|---|
| 3 mo
|
12 mo
|
>24 mo, CHIP+/+ | |||
| CHIP+/+ | CHIP−/− | CHIP+/+ | CHIP−/− | ||
| Plasma | 31.33 ± 4.01 | 32.50 ± 3.18 | 53.20 ± 10.05 | 75.00 ± 6.72* | 80.20 ± 13.82 |
| Brain | 1,373.59 ± 209.28 | 1,459.41 ± 80.71 | 1,819.20 ± 183.09 | 2,863.07 ± 119.87** | 2,015.18 ± 188.90 |
| Heart | 2,404.50 ± 100.50 | 2,698.00 ± 354.27 | 2,754.40 ± 676.62 | 3,339.67 ± 579.18* | 3,103.32 ± 456.36 |
| Liver | 3,287.66 ± 806.74 | 4,664.57 ± 444.64** | 4,631.00 ± 205.97 | 6,247.53 ± 541.58* | 2,866.39 ± 745.34 |
| Lung | 630.38 ± 131.51 | 674.10 ± 196.30 | 954.15 ± 226.03 | 1,563.05 ± 141.59* | 1,849.89 ± 329.65 |
a
Asterisks indicate significant differences of 8-isoprostane levels in CHIP−/− tissues compared to CHIP+/+ tissues of the same age group (*, P < 0.05; **, P < 0.01 [Student's t test]).