FIG. 3.

RPA stimulates FANCJ unwinding of G4 DNA. (A and B) FANCJ protein (0.3 nM) was incubated with 0.5 nM TP-G4 substrate in the absence (A) or the presence (B) of 1.5 nM RPA heterotrimer under standard helicase reaction conditions at 30°C for the indicated times. M, ³²P-labeled single-stranded oligonucleotide marker; +, present; −, absent; ss, ssDNA. (C) Quantitative analysis of FANCJ G4 helicase activity in the absence or presence of RPA or E. coli ssDNA binding protein (ESSB). The data shown are the averages of results from at least three independent experiments, with SD indicated by error bars.