FIG. 5.

FANCJ unwinds human telomeric G4 DNA. (A and B) The indicated FANCJ concentrations (lanes 2 to 7) were incubated with 0.5 nM 5′-15ntTeR2-G4 (A) or 5′-8ntTeR2-G4 (B) at 30°C for 15 min under standard helicase assay conditions. Lane 1, no-extract control; lane 8, ³²P-labeled single-stranded oligonucleotide marker (M). (C) Quantitative analysis of FANCJ helicase activity on 5′-15ntTeR2-G4 and 5′-8ntTeR2-G4. The data shown are the means of results from three independent experiments, with SD indicated by error bars.