FIG. 6.
FANCJ requires a 5′ ssDNA tail to unwind a G4 substrate derived from a human triplet repeat sequence. (A and B) The indicated FANCJ concentrations (lanes 2 to 7) were incubated with 0.5 nM 5′-tailed d(CGG)7-G4 (A) or 3′-tailed d(CGG)7-G4 (B) at 30°C for 15 min under standard helicase assay conditions. Lane 1, no-extract control; lane 8, 32P-labeled single-stranded oligonucleotide marker (M). (C) Quantitative analysis of FANCJ helicase activities on 5′-tailed d(CGG)7-G4 and 3′-tailed d(CGG)7-G4. The data shown are the means of results from three independent experiments, with SD indicated by error bars.