FIG. 2.

LSD1 associates with TLX in Y79 cells and in vitro. (A) Immunoprecipitation of nuclear extracts of Y79 cells, followed by Western blot (IB) analysis using anti-TLX, anti-PNR, and anti-LSD1 antibodies. Mouse immunoglobulin G (IgG) immunoprecipitate was used as a negative control. (B) Knockdown by LSD1 RNAi. Immunoprecipitation of nuclear extracts from control Y79 cells (shLacZ) and LSD1-depleted Y79 cells (shLSD1), followed by Western blot analysis using anti-TLX antibody. The confirmation of knockdown is shown in Fig. 4B. (C) In vitro interaction between TLX and LSD1. GST pull-down assays were performed with S³⁵-labeled LSD1 or its deletion mutants and bacterially expressed GST-TLX DBD and GST-TLX LBD fusion proteins. GST protein was used as a negative control. aa., amino acids.