FIG. 1.

CPC formation during maturation. (A) CPC subunit association. Extracts from immature (IM) and meta-II-arrested (MII) oocytes taken before (−) and after (+) immunodepletion with preimmune serum (Pre-Im) or anti-Aurora B antibodies were immunoblotted with antibodies against either INCENP, TD-60, Aurora B, Dasra A, or survivin, as indicated. The positions of molecular size markers are given on the right. (B) The Aurora B kinase activity of resting (Pro-I) oocytes or of oocytes treated with progesterone was measured at the indicated times with immunoprecipitated (IP) Aurora B by using histone H3 as a substrate as described in Materials and Methods. (C) Kinetics of CPC subunit accumulation. Progesterone (PG)-treated oocytes were homogenized at the indicated times, and extracts were immunoblotted with antibodies against INCENP, Aurora B, survivin, or the phospho-T-loop of Aurora B.