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. 2008 Mar 31;28(12):4196–4203. doi: 10.1128/MCB.00169-08

FIG. 2.

FIG. 2.

Effect of INCENP accumulation on oocyte maturation. (A) Antisense (AS) oligonucleotide ablation of INCENP. Oocytes were first injected with control oligonucleotides or INCENP AS oligonucleotides and then treated with progesterone. Extracts from immature (IM) and meta-II oocytes were then immunoblotted with antibodies against INCENP or Aurora B, as indicated. (B) Aurora B kinase activity was measured with Aurora B immunoprecipitates (IP) in uninjected oocytes (IM) or progesterone-treated meta-II oocytes that had previously been injected with control oligonucleotides or INCENP AS oligonucleotides, as indicated. Similar results were obtained in several independent experiments. (C) The level of histone H1 kinase activity was determined at the indicated times after GVBD in extracts from progesterone-treated oocytes injected with control oligonucleotides or AS oligonucleotides against INCENP. (D) Cyclin B2 levels after ablation of INCENP during maturation. Oocytes were injected with control oligonucleotides or INCENP AS oligonucleotides, incubated overnight, and then treated with progesterone. Maturing oocytes were crushed at the indicated time points after GVBD (meta-I). Extracts were then immunoblotted with anti-cyclin B1 or anti-cyclin B2 antibodies.