FIG. 6.

Reduced adipocyte differentiation in 3T3-L1 cells stably expressing Klf3. (A) 3T3-L1 preadipocytes were retrovirally infected with empty vector (EV), Klf3, or Klf3ΔDL and subjected to Oil Red O staining to assess for lipid accumulation at day 5 of differentiation. Macroscopic (upper panel) and microscopic (magnification, ×100; lower panel) views are shown. Images are representative of at least three independent experiments. (B) 3T3-L1 cells were retrovirally infected with EV, Klf3, or Klf3ΔDL and differentiated. Nuclear extracts were prepared and subjected to Western blot analysis using the indicated antibodies. All data are representative of at least three independent experiments.