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. 2008 Mar 10;28(10):3301–3312. doi: 10.1128/MCB.01542-07

FIG. 3.

FIG. 3.

The wobble mcm5 side chain in tRNAmcm5UCUArgand tRNAmcm5UCCGlyimproves reading of G-ending codons. (A) Wild-type (W303-1B), elp3Δ (UMY2843), tr(ccu)jΔ (UMY3137), and elp3Δ tr(ccu)jΔ (UMY3136) cells carrying the low-copy-number URA3 plasmid pRS316-tR(CCU)J were grown in liquid SC medium for 24 h. The cells were serially diluted, spotted onto SC plates and SC plates containing 5-fluoroorotic acid (5-FOA), and incubated for 3 days at 30°C. On 5-FOA-containing medium, only the cells that lost the URA3 plasmid were able to grow (5). The tR(CCU)J gene codes for tRNACCUArg. (B) The wild-type (W303-1B), elp3Δ (UMY2843), tg(ccc)dΔ tg(ccc)oΔ (UMY3304), and tg(ccc)dΔ tg(ccc)oΔ elp3Δ (UMY3320) strains were streaked on a YEPD (yeast extract, peptone, dextrose) plate and incubated at 30°C for 2 days. The tG(CCC) genes code for tRNACCCGly. wt, wild type.