FIG. 6.

Influence of the esterified methyl group of mcm⁵ side-chains on the decoding properties of tRNA. (A) The indicated strains (W303-1B, UMY3297, UMY3137, UMY3358, UMY3304, and UMY3360) were streaked on a YEPD (yeast extract, peptone, dextrose) plate and incubated at 30°C for 2 days. (B) The indicated strains (UMY3348, UMY3354, UMY3134, or UMY3345) were transformed with an empty high-copy-number (h.c.) LEU2 vector (10) or the same plasmid harboring a gene coding for the relevant U₃₄-containing tRNA [tE(UUC) or tQ(UUG)]. The transformants were grown in SC-Leu medium for 24 h, serially diluted, spotted onto SC-Leu plates and SC-Leu plates containing 5-fluoroorotic acid (5-FOA), and incubated at 30°C for 2 days. wt, wild type.