TABLE 2.
Steady-state levels of tRNAs with hypomodified wobble uridines
| tRNA speciesa | Relative tRNA levelb
|
||
|---|---|---|---|
| elp3Δ | tuc1Δ | trm9Δ | |
| tRNAncm5UmAALeu | 0.99 ± 0.13 | ND | ND |
| tRNAncm5UACVal | 0.83 ± 0.04 | ND | ND |
| tRNAncm5UGASer | 0.97 ± 0.11 | ND | ND |
| tRNAncm5UGGPro | 0.99 ± 0.20 | ND | ND |
| tRNAncm5UGUThr | 0.85 ± 0.11 | ND | ND |
| tRNAncm5UGCAla | 0.93 ± 0.12 | ND | ND |
| tRNAmcm5UCUArg | 0.95 ± 0.10 | ND | 0.79 ± 0.12 |
| tRNAmcm5UCCGly | 1.09 ± 0.07 | ND | 0.87 ± 0.08 |
| tRNAmcm5s2UUGGln | 0.98 ± 0.09 | 1.04 ± 0.12 | 0.90 ± 0.13 |
| tRNAmcm5s2UUULys | 0.96 ± 0.27 | 1.02 ± 0.14 | 0.88 ± 0.27 |
| tRNAmcm5s2UUCGlu | 1.02 ± 0.13 | 1.06 ± 0.17 | 0.80 ± 0.15 |
a
The tRNA symbol indicates the wild-type anticodon sequence.
b
The signal of respective tRNA species was normalized to the tRNAiMet signal and expressed relative to the corresponding value in the wild-type (wt) strain, which was set at 1. The values represent the average from the experiment shown in Fig. 2A and two additional independent experiments. The standard deviation is indicated. ND, not determined.