. 2008 Mar 10;28(10):3301–3312. doi: 10.1128/MCB.01542-07

TABLE 3.

In vivo aminoacylation levels of tRNAs with hypomodified wobble uridines

tRNA species^a	In vivo aminoacylation level (%)^c
	Experiment 1				Experiment 2
	wt	elp3Δ	tuc1Δ	trm9Δ	wt	elp3Δ	tuc1Δ	trm9Δ
tRNA_ncm⁵UmAA^Leu	83	87	ND	ND	58	67	ND	ND
tRNA_ncm⁵UAC^Val	88	92	ND	ND	86	86	ND	ND
tRNA_ncm⁵UGA^Ser	61	61	ND	ND	82	76	ND	ND
tRNA_ncm⁵UGG^Pro	69	76	ND	ND	46	52	ND	ND
tRNA_ncm⁵UGU^Thr	80	82	ND	ND	67	68	ND	ND
tRNA_ncm⁵UGC^Ala	80	79	ND	ND	53	62	ND	ND
tRNA_mcm⁵UCU^Arg	67	68	ND	65	39	45	ND	50
tRNA_mcm⁵UCC^Gly	84	87	ND	92	75	73	ND	80
tRNA_mcm⁵s²UUG^Gln	83	81	82	80	60	62	59	65
tRNA_mcm⁵s²UUU^Lys	79	81	86	84	61	56	63	61
tRNA_mcm⁵s²UUC^Glu^b	85	83	85	83	94	92	91	94
tRNA_i^Met	72	71	71	68	58	59	56	54

The tRNA symbol indicates the wild-type anticodon sequence.

Two different aminoacylated species were observed using the tRNA_mcm⁵s²UUC^Glu probe. The aminoacylation level for tRNA_mcm⁵s²UUC^Glu was probably overestimated since the signal for the slower-migrating nonaminoacylated form is in close proximity to the signals for the two aminoacylated forms.

wt, wild type; ND, not determined.