TABLE 4.
The wobble mcm5 group in an ochre suppressor tRNA promotes reading of UAA and UAG stop codons
| Termination codon | % Read-througha
|
Reduction (n-fold) in read-through (value for SUP4 /value for SUP4 elp3) | ||
|---|---|---|---|---|
| wt | SUP4 | SUP4 elp3Δ | ||
| UAA | 0.10 ± 0.02 | 29.49 ± 9.03 | 11.31 ± 1.63 | 2.6 |
| UAG | 0.12 ± 0.03 | 1.39 ± 0.24 | 0.31 ± 0.04 | 4.5 |
| UGA | 0.10 ± 0.02 | 0.09 ± 0.01 | 0.09 ± 0.02 | 1.0 |
a
The β-galactosidase activity from cells harboring the indicated nonsense reporter construct was expressed as a percentage of the activity obtained in the corresponding cells carrying the control construct. The values represent the averages from six independent transformants. The standard deviation is indicated.