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. 2008 Mar 17;28(10):3198–3207. doi: 10.1128/MCB.01435-07

FIG. 5.

FIG. 5.

PRMT5 is translocated into the nucleus through interaction with AJUBA and SNAIL. (A) Subcellular localization of AJUBA and PRMT5 in U2OS cells. The plasmids encoding Myc-AJUBA and Flag-PRMT5 were transiently transfected into U2OS cells, and immunofluorescent images were taken with confocal microscopy. AJUBA is predominately cytoplasmic when expressed alone, and PRMT5 is cytoplasmic with a distribution pattern similar to that of AJUBA (top panel). The coexpression of AJUBA and PRMT5 is shown in the bottom panel. (B) Individual expression of PRMT5, SNAIL, RFP-AJUBA, and RFP in U2OS cells. When expressed alone, the SNAIL protein is localized in the nucleus, while the RFP-AJUBA and PRMT5 proteins are in the cytoplasm; RFP alone is found in both cytoplasm and nucleus. (C) The coexpression of RFP and SNAIL does not affect their localization. (D) The coexpression of AJUBA and SNAIL affects both of their localizations: the SNAIL protein is retained in the cytoplasm, and concomitantly, a significant amount of the AJUBA protein is localized in the nucleus. (E) The coexpression of PRMT5, RFP, and SNAIL reveals no apparent effect on either localization. (F) The coexpression of RFP-AJUBA, SNAIL, and PRMT5 results in a significant amount of the PRMT5 protein being relocated to the nucleus and colocalized with nuclear SNAIL and AJUBA.