FIG. 6.

The AJUBA protein can simultaneously immunoprecipitate SNAIL and PRMT5. (A) Coimmunoprecipitation of the SNAIL, AJUBA, and PRMT5 proteins. The plasmids encoding Myc-AJUBA, Flag-SNAIL, and Flag-PRMT5 were transiently transfected into HEK293 cells, and immunoprecipitation (IP) was done with anti-Myc antibody and Western blotting (Wb) with anti-Flag antibody. The asterisk shows nonspecific bands. +, present; −, absent; α, anti. (B) Western blots show the eluted SNAIL, AJUBA, and PRMT5 proteins from the Superose 6 sizing column. Whole-cell extract (8 mg) was prepared from HEK293 cells transiently expressing Myc-AJUBA, Flag-SNAIL, and Flag-PRMT5 and was loaded onto a Superose 6 gel filtration column. Molecular sizes are shown on the left. MM, molecular mass.