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. 2008 Mar 17;28(10):3198–3207. doi: 10.1128/MCB.01435-07

FIG. 8.

FIG. 8.

Modulation of AJUBA and PRMT5 in p19 cells results in upregulation of E-cadherin expression. (A) Western blotting results show that siRNA targeting AJUBA can stimulate E-cadherin gene expression in p19 cells. Molecular sizes are shown on the left. The asterisk shows nonspecific bands. (B) Reverse transcriptase PCR (RT-PCR) analysis of the E-cadherin mRNA level in siAjuba p19 cells. Molecular sizes are shown on the left. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (C and D) The methyltransferase inhibitor MTA stimulates E-cadherin expression in p19 cells (C), and similarly, siRNA knockdown of PRMT5 in p19 cells results in the upregulation of E-cadherin gene expression (D), shown by the results of Western blotting.