FIG. 6.

Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to the cell-cell junctions during the Ca²⁺ switch. (A) MTD-1A cells transfected with control RNA or actinin-4 siRNA were subjected to a Ca²⁺-switch assay and then immunostained with anti-JRAB/MICAL-L2 antibody at 0, 4, and 6 h after Ca²⁺ restoration. Bar, 20 μm. (B) MTD-1A cells were transfected with control RNA or JRAB/MICAL-L2 siRNA and subjected to Western blotting analysis using anti-JRAB/MICAL-L2 and anti-β-actin antibodies. (C) MTD-1A cells transfected with control RNA or JRAB/MICAL-L2 siRNA were subjected to a Ca²⁺-switch assay and then immunostained with anti-actinin-4 antibody at 0, 4, and 6 h after Ca²⁺ restoration. Bar, 20 μm. The results shown in panels A to C are representative of three independent experiments.