Skip to main content
. 2008 Feb 25;28(10):3139–3150. doi: 10.1128/MCB.01469-07

FIG. 3.

FIG. 3.

Constitutive expression of RelB is sufficient to restore LTβR-induced RelB activation in rela−/− MEFs. (A and C) Immunoblotting to reveal p100 (A) or RelB (C) expression from transgene in rela−/− MEFs. (B and F) EMSA to examine NF-κB DNA binding activity in response to LTβR stimulation in rela−/− MEFs that expresses p100 (B) or RelB (F) from transgene. (D) Steady-state level of NF-κB2 mRNA in rela−/− MEFs in the absence or presence of the retroviral RelB transgene was analyzed by RPA. (E) Immunoblotting to examine processing of p100 and generation of p52 upon LTβR stimulation in rela−/− MEFs in the absence or presence of the retroviral RelB transgene. (G) Supershift analysis to examine the composition NF-κB DNA binding activity induced upon LTβR stimulation in rela−/− MEFs that expresses RelB transgene. α, anti.