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. 2008 Feb 25;28(10):3139–3150. doi: 10.1128/MCB.01469-07

FIG. 4.

FIG. 4.

Constitutive IKKβ activity determines LTβR-induced activation of RelB/NF-κB. (A) NF-κB DNA binding activities induced upon LTβR stimulation in MEFs of the indicated genotypes were analyzed by EMSA. (B) Steady-state levels of NF-κB2 and RelB mRNA in wild-type and ikkβ−/− MEFs was examined by RPA. (C and D) Immunoblotting to reveal the steady-state level of p100 and RelB protein in MEFs of the indicated genotypes. (E) NF-κB DNA binding activity induced by LTβR stimulation in ikkβ−/− MEFs expressing transgenic wild-type (lanes 1 to 4), inactive (lanes 5 to 8), or constitutively active forms (lanes 9 to 12) of IKKβ or ectopic RelB protein (lanes 13 to 16). (F) Supershift analysis to examine the composition of NF-κB DNA binding activity induced upon LTβR stimulation in the ikkβ−/− MEFs reconstituted with the indicated retroviral transgene. α, anti.