FIG. 5.

Requirement of both nfkb1 and nfkb2 gene products for induction of RelB target genes and NF-κB/RelB DNA binding activity in response to LTβR signaling. (A and B) Expression of BLC and SLC genes upon LTβR stimulation in primary MEFs of the indicated genotypes. (C) NF-κB DNA binding activities induced by LTβR stimulation in primary MEFs of the indicated genotypes were examined by EMSA. The presence of RelB in single nfkb1^−/− and nfkb2^−/− knockout cells perturbs NF-κB RelA activation. (D) Supershift analysis to reveal the composition of NF-κB DNA binding activity induced upon LTβR stimulation in the MEFs of the indicated genotypes. α, anti.