FIG. 2.

p110 CUX1 cooperates with E2F1 in the activation of the DNA Pol α gene promoter in a binding site-dependent manner. (A and C) Hs 578T cells were transfected with wild-type or mutant DNA Pol α (−65/+47)/luciferase reporter constructs and the indicated vectors expressing CUX1 or E2F1. Cytoplasmic extracts were prepared and processed to measure luciferase activity. Results were expressed as relative light units and were normalized to β-galactosidase activity from an internal control. The means of three transfections are shown. Results are representative of three separate experiments. (B) The diagram and DNA sequence show the position of the linker scanner and insertion mutations within the DNA Pol α gene promoter. The sequences of CUX1 and E2F sites are underlined. Stimulation is expressed relative to the wild-type reporter construct. The transcription start site is indicated with an arrow.