FIG. 6.

shRNA-mediated inhibition of CUX1. T98G cells were infected with pLVTHM lentivirus expressing CUX1 shRNA or with the empty vector. EGFP-expressing cells were subjected to fluorescence-activated cell sorting 3 days postinfection and cultured for 5 days. Nuclear extracts were separated by SDS-polyacrylamide gel electrophoresis and analyzed by Coomassie blue staining and immunoblotting with CUX1 antibodies.