TABLE 3.
Inhibition of CUX1 expression reduces the recruitment of E2F1 to endogenous gene targetsa
| Gene | Enrichment
|
|||
|---|---|---|---|---|
| E2F1
|
E2F2
|
|||
| Vector | Cux1 shRNA | Vector | Cux1 shRNA | |
| CCNA2 | 9.2 | 2.9 | 8.7 | 2.8 |
| MCM7 | 16.5 | 9.0 | 20.2 | 8.5 |
| ORC1L | 8.1 | 3.4 | 9.2 | 3.6 |
| POLA | 11.5 | 5.6 | 4.3 | 2.5 |
| PMS1 | 7.7 | 2.5 | 3.8 | 2.1 |
a
T98G cells were infected with pLVTHM lentivirus expressing CUX1 shRNA or with the empty vector. EGFP-expressing cells were sorted by fluorescence-activated cell sorting 3 days postinfection, cultured for 5 days, and then processed for ChIP assay with E2F1 antibodies. Quantitative PCR results are expressed as enrichment relative to no antibody ChIP control and normalized for enrichment of G6PDH. Expression of CUX1 is shown in Fig. 6.