FIG. 5.

Emi1 mediates RASSF1A depletion-induced G₁ arrest. (A) HeLa cells were transfected and immunoblotted for indicated proteins as described for Fig. 2A. (B) Cells were transfected with the indicated plasmids. At 48 h posttransfection, whole-cell lysates were immunoblotted for the indicated proteins. (C) BrdU incorporation was assayed as described for Fig. 2D. Representative micrographs are shown. (D) FACS analysis of HeLa cells transfected with the indicated siRNAs. Population distributions are shown relative to DNA content. Results are representative of three independent experiments. Ctrl, control; DAPI, 4′,6′-diamidino-2-phenylindole.